Plasmid_Backbone

Part:BBa_K4212027:Design

Designed by: Fontaine Gibbs   Group: iGEM22_Imperial_College_London   (2022-09-30)


STK-1A-sfGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1028
    Illegal XbaI site found at 1043
    Illegal PstI site found at 2106
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1028
    Illegal PstI site found at 2106
    Illegal NotI site found at 1034
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1028
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1028
    Plasmid lacks a suffix.
    Illegal PstI site found at 2106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1028
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1043
    Illegal PstI site found at 2106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2084
    Illegal BsaI.rc site found at 1066
    Illegal SapI.rc site found at 1250


Design Notes

sfGFP dropout cassette inserted within the MCS of backbone, flanking with BsaI and BsmBI sites. Dropout cassette is replaced by target sequences, which enables to verify the insertion of target sequences if transformants do not express sfGFP.


Source

Synthetic construct

References